Plant Genome II Conference
Town & Country Conference Center, San Diego, CA, January, 1994.
PG-II: MAPPING THE MAIZE GENOME WITH YACs
MAPPING THE MAIZE GENOME WITH YACs
Keith Edwards, Heather Rawles, Allan Daly and Keith Rufener,
Plant Biotechnology Section, Zeneca Seeds, Jealott's Hill
Research Station, Bracknell, Berkshire, UK RG12 6EY.
We have constructed a maize YAC library containing 79,000
clones with an average insert size of 145 kb. Using both a PCR
screening protocol and robot aided high density colony
hybridisation we have begun to isolate and characterise numerous
sequences from throughout the genome. Our preliminary studies
have shown that YAC's prepared from maize genomic DNA are
difficult to characterize due to the presence of large numbers
of repeat sequences. Although these repeats can interfere with
both the mapping of individual YAC's and chromosome walking we
have shown that it is possible to use maize YAC's to physically
link markers which can be separated on the genetic map. We are
now using repeat sequences to catalog individual YAC's with
respect to the Organisation of the different repeats within the
genome. We believe that these studies will eventually lead to a
better understanding of the role which repeats play in the
organisation and evolution of a complex plant genome.
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