Plant Genome II Conference
Town & Country Conference Center, San Diego, CA, January, 1994.
PG-II: MICROSATELLITES AS A MOLECULAR MARKER SYSTEM FOR WHEAT
MICROSATELLITES AS A MOLECULAR MARKER SYSTEM FOR WHEAT
Katrien Devos, Glenn Bryan, Angela Collins, Jim Smith, Pauline
Stephenson, and Mike Gale, Cambridge Laboratory, Colney, Norwich
NR4 7UJ, UK
The potential applications of genetic maps in plant breeding
have long been recognized. In wheat, detailed RFLP-based maps
containing some 1000 loci have been constructed. However, the
relatively low level of polymorphism detected by RFLP probes, and
the localization of recombination in the distal chromosome
regions, resulting in a low genetic density of probes in these
regions, has prompted a search for new types of markers to
complement the RFLP loci. A possible source of polymorphism is
length variation in microsatellite sequences. A study of a
series of microsatellites present in the a,B-gliadin, y-gliadin
and low molecular weight glutenin genes revealed substantial
polymorphism in samples of closely related varieties. Details
about the basis of the detected variation, the specificity of
microsatellite markers and their map position will be presented.
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