Plant Genome II Conference
Town & Country Conference Center, San Diego, CA, January, 1994.
PG-II: GENE PRODUCT AMOUNTS AND MACROSCOPIC TRAITS DISPLAY CONTRASTED
VALUES FOR QTL PARAMETERS IN MAIZE
GENE PRODUCT AMOUNTS AND MACROSCOPIC TRAITS DISPLAY CONTRASTED
VALUES FOR QTL PARAMETERS IN MAIZE.
Catherine Damerval, Alexandrine Maurice, Agnes Leonardi,
Jean-Marc Josse and Dominique de Vienne, Station de Genetique
Vegetale, INRA/CNRS URA 1492/Universite Paris XI, La Ferme du
Moulon, 91190 Gif-sur-Yvette, France.
Seventy-two anonymous polypeptide spots resolved using
silver-stained high-resolution two-dimensional electrophoresis
gels of maize coleoptile extracts were automatically quantified
for every individual of an F2 progeny between distant lines. A
complete genetic linkage map, including 70 RFLP and 39 protein
markers, allowed us to map Mendelian factors underlying
quantitative variation (QTLS) for 42 polypeptides. One to 5
unlinked chromosomal regions were found to affect single
polypeptides, 27% of which resulted in more than a doubling of
the polypeptide spot intensity. Dominance was observed for half
of the factors, with high amount dominant over low amount in most
cases, which is consistent with the observation that the relative
abundance of proteins in Fl hybrids is in average deviated to the
high parental values. Epistatic interactions were shown to be
involved in the control of 14% of the proteins. These features
contrast sharply with the observations from 20 agromorphological
traits measured in the same progeny, where limited substitution
and dominance effects, and no epistatic interaction, were found.
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