Plant Genome II Conference
Town & Country Conference Center, San Diego, CA, January, 1994.
PG-II: RESULTS OF EARLY- VS. LATE-GENERATION SELECTION IN BACKCROSS
BREEDING USING RFLP MARKERS
RESULTS OF EARLY- VS. LATE-GENERATION SELECTION IN BACKCROSS
BREEDING USING RFLP MARKERS.
Yan-San Chyi, Jennifer Taylor, Kurt Kellesvig, and Larry Sernyk,
Mycogen Plant Sciences, Madison Laboratories, 5649 E. Buckeye
Rd., Madison, WI 53716.
RFLP marker technology can be used to accelerate conversion
breeding by identifying individuals with highest recurrent genome
content for advancement in each backcross generation. Several
variations of the use of RFLP markers to assist in selection have
been tested in our backcross breeding program. Reported here are
observations from the comparison of two regimes where
RFLP-assisted selection was not employed in the first vs. the
second backcross generations. The potential progress to be made
by, and thus the necessity of, RFLP-based selection depends on
the detectable polymorphism between the donor and the recurrent
parents involved in the backcross program. The technology is
more powerful when less polymorphism is involved, such as in
closely related lines and in advanced generations of
backcrossing. With RFLP marker analysis to ensure recurrent
genome recovery, individuals with the best expression of the
introduced traits can be selected for advancement in each
generation.
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