Plant Genome II Conference
Town & Country Conference Center, San Diego, CA, January, 1994.
PG-II: CONSTRUCNON AND UTILIZATION OF A DNA MARKER LINKAGE MAP OF
RAPESEED
CONSTRUCNON AND UTILIZATION OF A DNA MARKER LINKAGE MAP OF
RAPESEED.
Yan-San Chyi, Mary Hoenecke, and Larry Sernyk, Mycogen Plant
Sciences Madison Laboratories, 5649 E. Buckeye Rd., Madison, WI
53716.
A complete genetic linkage map can be used to locate
important genes and to analyze individual plant genomes. This
map can be applied as a tool in the selection of desired
recombinants in a plant breeding program and thus speed up the
breeding cycle. An RFLP restriction fragment length
polymorphism) DNA marker linkage map for the rapeseed species
Brassica rapa has been constructed. This map contains 360 marker
loci covering the 10 chromosomes of this species with a total
distance of 1876 genetic recombination units. This is one of the
largest known plant RFLP maps based on nuclear DNA amounts. The
B. rapa RFLP map has been used to map three genes associated with
desirable quality traits. The RFLP markers closely linked to
these quality genes can be used to assist in the selection of
individuals which contain these genes. The B. rapa RFLP map is
also being employed to genetically "fingerprint" individuals in
order to determine their genomic constitution and thus allow
selection of desired recombinants. Applying selection based on
RFLP markers has reduced the breeding time of new varieties by
25% or more.
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