Plant Genome II Conference
Town & Country Conference Center, San Diego, CA, January, 1994.
PG-II: AA GENOME SPECIFIC PROMOTER LIKE DNA SEQUENCE FROM INDICA RICE
(IR36) GENOME
AA GENOME SPECIFIC PROMOTER LIKE DNA SEQUENCE FROM INDICA RICE
(IR36) GENOME.
Abhijit Datta, DK Mukhopadhyay and Rajat K. Chaudhuri. Molecular
Biology Laboratory, Botany Department, Calcutta University,
Calcutta-700019, INDIA.
Rice genome is small enough (0.6 pg) for molecular genetic
studies particularly in the background that enough data have been
accumulated with the initiative of rice genome projects. We have
cloned a 700 base pair Mbo I repeat from IR36 rice genome which
is non-ribosomal in nature. During characterisation and
sequencing the clone (pR36VII) shows the presence of TATA
sequence followed by a ATG codon. A significant portion of the
clone thus represents a putative promoter sequence. Restriction
analysis shows recognition sequences for 13 restriction
endonucleases. The distribution pattern of this clone indicates
that the said sequence is AA genome specific and is absent from
Aeries officinalis, (CC) genome. Also our data suggest that the
same could be further dissected for a successful RFLP probe. The
copy number of this sequence indicates it as a middle repetitive
DNA sequence.
Return to Previous Page or Intl-PAG Homepage