Plant Genome II Conference
Town & Country Conference Center, San Diego, CA, January, 1994.
PG-II: SIMPLE SEQUENCE REPEAT DNA MARKERS FOR GENOME MAPPING AND
GENOTYPE IDENTIFICATION
SIMPLE SEQUENCE REPEAT DNA MARKERS FOR GENOME MAPPING AND
GENOTYPE IDENTIFICATION.
Perry B. Cregan, Mahinur S. Akkaya, Jiang Rongwen, Soybean and
Alfalfa Research Lab, USDA, ARS, Beltsville, MD 20705, Arvind A.
Bhagwat, Dept. of Agronomy, University of Maryland, College
Park, MD 20742; and Uri Lavi, Inst. of Horticulture, ARO, The
Volcanic Center, Bet-Dagan, Israel.
Simple Sequence Repeat (SSR) or microsatellite DNA markers
have become the marker of choice by human and other mammalian
geneticists. Characteristics of SSR markers that have resulted
in their rapid acceptance include:
Highly informative nature
Abundance
Co-dominant inheritance
High level of reproducibility
PCR amplification
No probe maintenance and distribution.
We and other groups have demonstrated the presence of SSRs in a
number of plant species including soybean, maize, rice, alfalfa,
Brassica, mango, avocado, and apple. Among a group of 96 diverse
soybean genotypes we have identified SSR loci with as many as 29
alleles. In this group of genotypes, Polymorphism Information
Content (PIC) values of SSR markers have ranged from 0.60 to
0.95. Using a selected set of markers even closely related
genotypes can often be distinguished. Mapping of these loci in
two soy bean populations suggests that SSR loci are well
distributed throughout the soybean genome. Linkages with a
number of RFLP and morphological markers have also been detected
in one mapping population. If other plant species possess levels
of polymorphism comparable to soybean SSR markers should be as
useful to plant geneticists as they have proven to be in
mammalian systems.
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