Plant Genome II Conference
Town & Country Conference Center, San Diego, CA, January, 1994.
PG-II: DIFFERENTIAL RESPONSES OF INDICA AND JAPONICA RICE GENOTYPES TO
TISSUE CULTURE FOR SALT-TOLERANCE
DIFFERENTIAL RESPONSES OF INDICA AND JAPONICA RICE GENOTYPES TO
TISSUE CULTURE FOR SALT-TOLERANCE.
AB Mandal 1, AK Bandopadhyay 1, Ila Chaudhuri 2 and Rajat K.
Chaudhuri 2. 1 Central Agricultural Research Institute, Port
Blair 744101, Andamans, India; 2 Molecular Biology Lab., Botany
Department, Calcutta University, Calcutta-700019, India.
Three genetically divergent genotypes of rice (Oryza sativa)
namely, Fuzisaka 3 (japonica rice), Pokkali (tall indica rice -
salt tolerant) and B116 (improved indica rice-medium salt
tolerant), were subjected to callusing on MS medium, followed by
sodium chloride stress before plantlet regeneration. A total
number of 118 salt-tolerant regenerates were planted and screened
for salt tolerance in the humid tropical and saline condition, in
huge plots in Port Blair, and identified as SC1 variants. More
than 2000 plantlets were screened as second generation somaclonal
(SC2) variants after growing them in huge plots. Regeneration
data show that genotypically indica cultivars particularly native
variety Pokkali are more prone to somaclonal variation than that
observed in Fuzisaka 3. Occurrence of albino plantlets, which
are common after salt-stress in tissue culture, was found to be
less in indica cultivars. Also they showed significant reduction
in plant height, days to flower and increase in grain length.
Though few SC2 regenerated plants could not withstand saline
condition (revertants ?), but some variants showed luxuriant
plant growth. Also, sterility percent was found to be very high
(63%) in Fuzisaka 3. Pokkali showed overall improvements in
agronomic traits. Molecular basis for these variation is being
subjected to experimentation with DNA probes available to RKC.
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