Plant Genome II Conference
Town & Country Conference Center, San Diego, CA, January, 1994.
PG-II: SINGLE TREE GENETIC LINKAGE MAP FOR DOUGLAS-FIR
SINGLE TREE GENETIC LINKAGE MAP FOR DOUGLAS-FIR.
E. Jason Broome and John E. Carlson, The Biotechnology Laboratory
and Department of Forest Sciences, University of British
Columbia, Vancouver, British Columbia, Canada
A Random Amplified Polymorphic DNA (RAPD) based genetic
linkage map for a single Douglas-fir tree (Pseudotsuga menziesii
var. menziesii (coastal), N = 13) was constructed using haploid
DNA isolated from the megagametophyte tissue of individual seeds.
One hundred and sixty segregating RAPD marker loci which
conformed to the expected Mendelian ratio were identified. The
PC-compatible version of Mapmaker/Exp 3.0 software (courtesy S.
Lincoln) was used to establish linkage relationships and linear
orders of the markers at a LOD value of 3.0. One hundred and
twenty-five of the segregating markers were found to be
distributed among 25 linkage groups covering 1924.3 cM of the
Douglas-fir genome. The remaining 35 markers were unlinked.
Return to Previous Page or Intl-PAG Homepage