PAG-II Plant Genome II Conference

Town & Country Conference Center, San Diego, CA, January, 1994.


PG-II: MAPPING QTL FOR SEED PROTEIN AND OIL IN SOYBEAN

MAPPING QTL FOR SEED PROTEIN AND OIL IN SOYBEAN.

E.C. Brummer 1, G.L. Graef 2, J. Orf 3, J.R. Wilcox 4, and R.C. Shoemaker 5. 1 Dept. of Agronomy, Iowa State Univ., 2 Univ. of Nebraska, 3 Univ. of Minnesota, 4 USDA-ARS-FCR, Purdue Univ., 5 USDA-ARS-FCR, Iowa State Univ., Ames, IA 50011.


Soybean is one of the most important sources of vegetable oil and protein meal in the world. We are conducting a long-range study in an attempt to map chromosomal segments on the ISU/USDA public soybean RFLP map which are associated with the expression of these traits. Eight F2 populations have been derived from crosses between Glycine max lines. Advanced generations have been grown in the field in Minnesota, Indiana, and Nebraska for two years. Results from regression analyses show that several RFLP loci are associated with QTL in each population. Common QTL among the populations have also been observed in some cases. Similarities in QTL location have also been noted with results from a G. max x G. soia population (Diets et al, TAG, 1992). In general, RFLP loci detect QTL for both protein and oil, but some loci appear to be linked only to one trait or the other. This suggests that selection may be possible to concurrently increase both protein and oil, something which has eluded traditional breeding programs. Additionally, the use of multiple populations in mapping studies providing us with information on duplicate loci and new, informative probe/enzyme combinations. This will make the public molecular map more useful in breeding programs which use cultivated (G. max) soybean accessions as parents.


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