PAG-II Plant Genome II Conference

Town & Country Conference Center, San Diego, CA, January, 1994.


PG-II: STUDY OF A LEAF RUST RESISTANCE GENE CARRIED BY A xMV CHROMOSOME OF Aegilops ventricosa

STUDY OF A LEAF RUST RESISTANCE GENE CARRIED BY A xMV CHROMOSOME OF Aegilops ventricosa.

A. Bonhomme(l), J. Jahier(2), P. Nicolas(3), M. Bernard (1) (1) INRA, 63039 Clermont-Ferrand cedex, France. (2) INRA, 35650 Le Rheu, France. (3) Universite Blaise Pascal, 63000 Clermont-Ferrand, France.


A leaf rust resistance gene carried by a chromosome of the Aegilops ventricosa MV genome has been introduced in the hexaploid wheat Moisson. The constitution of the chromosome xMV found in a wheat alien chromosome addition line (Moisson + xMV), a deleted addftion line (Moisson + xMV deleted for the Lr gene) and a substitution line (Mooisson 6D, + xMV) was studied. The part of this chromosome conferring resistance to leaf rust was identified using 68 wheat probes.

This RFLP analysis showed that:

--the xMV found in the addition line v260 was originated from Aegilops ventricosa N 10,

--xMV was a rearranged chromosome, constituted with a large part of the 6MV (deleted for a piece of its short arm) and a fragment of a chromosome 2 (2MV or 2DV) of Aegilops ventricosa translocated on the long arm of the 6MV,

--a chromosomal segment of the xMV, composed of 2 loci (PSR 150, PSR 933) and of the Lr gene, was introduced in the chromosome 2A of Moisson by homoeologous recombination (in the line 22).


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