Plant Genome II Conference
Town & Country Conference Center, San Diego, CA, January, 1994.
PG-II: TRANSFERRING INFORMATIVE MOLECULAR MARKERS BETWEEN BARLEY
(HORDEUM VULGARE L.) AND WHEAT (TRITICUM AESTIVUM L.)
TRANSFERRING INFORMATIVE MOLECULAR MARKERS BETWEEN BARLEY
(HORDEUM VULGARE L.) AND WHEAT (TRITICUM AESTIVUM L.)
Tom Blake, Luther Talbert and Dina Kadyrzhanov. Dept.
of Plant & Soil Sci. and Dept. of Animal Sci., Montana
State Univ., Bozeman, MT 59717.
The utility of molecular markers depends upon a combination
of their informativeness (the number of alleles/locus and the
distribution of those alleles), their reliability and their ease
of use. Restriction fragment length polymorphisms have proven
their utility in both reliability and informativeness and have
been the workhorse markers of map development in the small
grains. Our project is attempting to generate markers with
similar informativeness and reliability, but improved ease of
use. We have sequenced clones from the ITMI and NABGMP mapping
project, designed primer sets which amplify sequences homologous
to these sequences and utilized internal restriction site
polymorphisms or insertion/deletion events to provide
information. In this poster we present analyses of
informativeness of the amplified markers, and describe the
process of development of genome-specific single copy informative
markers for wheat.
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