Plant Genome II Conference
Town & Country Conference Center, San Diego, CA, January, 1994.
PG-II: DETECTION OF BARLEY POWDERY MILDEW RESISTANCE GENES USING RFLP
AND MICROSATELLITE DNA MARKERS
DETECTION OF BARLEY POWDERY MILDEW RESISTANCE GENES USING RFLP
AND MICROSATELLITE DNA MARKERS.
Ruslan M. Biyashev 1, M. A. Saghai Maroof 1, and Qifa Zhang 2,
1 Department of Crop and Soil Env. Sci., Virginia Polytech. Inst.
& State Univ., Blacksburg, VA 24061-0404; 2 Biotechnology Center,
Huazhong Agric. Univ., Wuhan, China
Powdery mildew is one of the most important diseases of
barley. Several loci conditioning resistance to this disease
have been reported previously. The objective of this study was
to use RFLPs and SSRs (simple sequence repeats or
microsatellites) to identify chromosomal regions containing genes
for powdery mildew resistance. A set of eight parental lines and
their 28 F1 crosses from a barley diallel study was inoculated
with each of the five isolates of Erysiphe graminisf hordei. The
parental lines were also screened for 89 RFLP and SSR markers
covering over 1100 cM of the barley genome. A total of 28 RFLP
and SSR markers detected significant effects of resistance to at
least one of the five powdery mildew isolates. Most of the loci
showed significant resistance effects to all five isolates.
Highly significant effects were revealed by markers on the barley
chromosomes 2, 4, 5, 6, and 7. The map location for many of
these marker loci correspond well with the chromosomal locations
of the known powdery mildew resistance genes from previous
reports. Results from this study further indicate that
marker-based diallel analysis can be used to detect the effect of
a specific gene as well as to determine the approximate map
position of the locus of interest if the linkage map is
adequately represented by the markers used in the survey.
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