PAG-II Plant Genome II Conference

Town & Country Conference Center, San Diego, CA, January, 1994.


PG-II: A GENETIC MAP OF COMMON BEAN TO LOCALIZE SPECIFIC RESISTANCE GENES AGAINST ANTHRACNOSE

A GENETIC MAP OF COMMON BEAN TO LOCALIZE SPECIFIC RESISTANCE GENES AGAINST ANTHRACNOSE.

Anne-Francoise Adam-Blondon, Mireille Sevignac, Michel Dron, Biologie moleculaire vegetale, Bat. 430, Universite Paris-Sud, 91405 Orsay-cedex, France.


One hundred and fifty-three molecular markers (51 restriction fragment length polymorphism, 90 random amplified polymorphic DNA, two sequence characterized amplified regions) were used to construct a genetic map covering 571.5cM of the bean genome. Two resistance genes towards anthracnose (Are and RVI), a dominant gene for nuclear male-sterility (Ms8) and a pod architecture character (Sgou) were also localized. This map was established by using two backcross populations (BC1) of 82 and 46 individuals, derived from a cross between two european bean cultivars: EO2 and Corel. Seven percent of the markers showed distortions of segregation and mapped to three regions. Clusters involving two to ten markers were observed in every linkage group. The possible origin of these clusters is discussed. Eighteen common markers to an already reported bean linkage map (GEPTS, P., NODARI, R., TSAI, S.M., KOINANGE, R.M.K., LLACA, V., GILBERTSON, R. and GUZMAN, P. 1993. Linkage mapping in common bean. Annu. Rept. Bean Improv. Coop., 36: 24-38) allowed to establish a preliminary correspondence between the two maps. The establishment of an integrated bean map and some applications of such a map are discussed.


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