PAG-II Plant Genome II Conference

Town & Country Conference Center, San Diego, CA, January, 1994.


PG-II: GENETIC DISSECTION OF QUANTITATIVE TRAITS IN RICE USING MOLECULAR MARKERS

GENETIC DISSECTION OF QUANTITATIVE TRAITS IN RICE USING MOLECULAR MARKERS.

Masahiro Yano, Department of Crop Breeding, Hokuriku National Agricultural Experiment Station, Joetsu, Niigata 943-01 Japan


Molecular markers make it possible to identify chromosomal regions underlying important agricultural traits, especially quantitative traits. This study employed the high density molecular map and markers (nearly 800 markers) for localization of the quantitative trait loci (QTLs) in rice. Genetical analyses have been carried out based on the F2 population between a Japonica variety, Nipponbare and an Indica variety, Kasalath. Major seven morphological traits such as, culm length, pence length, number of panicles, total number of seeds per panicle, awn length, seed shattering habit and heading date were analyzed. Transgressive segregation was observed for all of the traits in the F2 population. The thirty six putative chromosomal regions (LOD>2.0) were detected by the interval mapping for all the traits. Some traits had single QTL with the significant LOD score independently located in the chromosomal regions. Whereas the estimated QTL area for some traits showed wide area of one chromosomal region and showed more than one peak of the LOD score, indicating the existence of two or more putative QTLS. Under these circumstances, it was not possible to locate precisely each QTL on the chromosomal regions. The analysis of near isogenic lines or backcrossed inbred lines derived from this cross combination would be necessary for the fine mapping of these QTLs and estimation of their effects on the phenotypes.


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