Plant Genome II Conference
Town & Country Conference Center, San Diego, CA, January, 1994.
PG-II: SEQUENCE ANALYSIS OF THE MITOCHONDRIAL GENOME OF ARABIDOPSIS
THALIANA
SEQUENCE ANALYSIS OF THE MITOCHONDRIAL GENOME OF ARABIDOPSIS
THALIANA
Michael Unseld, Petra Brandt, Mathieu Klein and Axel Brennicke,
Institut fur Genbiologische Forschung GmbH, IhnestraBe 63, 14195
Berlin, Germany.
The complete mitochondrial genome of the flowering plant
Arabidopsis thaliana has been mapped by more than 300 overlapping
cosmid clones and is currently analyzed by sequencing. The
hypothetical "master molecule" contains 400 kb in a circular
arrangement. This arrangement contains two sets of actively
recombining repeats present in inverted and in directly repeated
directions. Cloned fragments of sequence arrangements across the
repeats confirm the presence of all possible different
arrangements in the mitochondrial DNA. Presently about 65% of
the genome have been sequenced within different regions. In the
sequences determined up to now about 50 genes have been
identified. Genes encoded in the mitochondrial genome of
Arabidopsis include informations for rRNAs, tRNAs, a number of
subunits in the respiratory chain complexes, ribosomal proteins
and polypeptides involved in the biogenesis of cytochrome c
complexes. Some of the genes are interrupted by one or more
group 11 introns. All of the protein coding genes analyzed so
far in this respect are altered by RNA editing in their
transcripts. Many smaller sequence repeats of coding and
non-coding regions as well as several pseudogenes and chloroplast
derived DNA sequences are present in the mitochondrial genome of
Arabidopsis.
Return to Previous Page or Intl-PAG Homepage