Plant Genome II Conference
Town & Country Conference Center, San Diego, CA, January, 1994.
PG-II: GENETIC CONTROL OF RECOMBINATION RATES IN MAIZE. I. VARIABILITY
AMONG LATIN AMERICAN RACES AND TEOSINTE
GENETIC CONTROL OF RECOMBINATION RATES IN MAIZE. I. VARIABILITY
AMONG LATIN AMERICAN RACES AND TEOSINTE.
Claire G. Williams, Charles W. Stuber, William F. Thompson &
Major M. Goodman, Departments of Genetics, USDA-ARS, Botany &
Crop Science, North Carolina State University, Raleigh North
Carolina 27695.
Use of saturated genetic maps and marker-assisted selection
has renewed interest in identifying modifier genes to break up
blocks of donor segments which do not readily recombine.
Molecular markers are used to introgress desirable chromosomal
segments from the donor into the recipient while simultaneously
selecting against undesirable segments of the donor genome.
Ultimately, our goal is to develop maize lines with enhanced or
restricted rates of recombination.
The purpose of this first study was to compare rates of
recombination across races of maize and teosinte. Testcrosses
were constructed using multiple marker stocks which are
homozygous for low-frequency isozyme allelic variants Data are
presented for Chromosome I stocks testcrossed to seven Latin
American maize races, Zea diploperennis and one domestic maize
inbred, B73. There is wide variability in recombination
fractions among exotic races of maize on chromosome I Segregation
distortion patterns also differ widely Studies are in progress to
i) experimentally test for genetic control of recombination using
these chromosomal testcross stocks, and maize meiotic mutants,
ii) develop probes for mapping these genes using subtractive
hybridization methods
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