PAG-II Plant Genome II Conference

Town & Country Conference Center, San Diego, CA, January, 1994.


PG-II: EVOLUTION OF DIVERSE PATTERNS OF ACTIN GENE REGULATION IN HIGHER PLANTS

EVOLUTION OF DIVERSE PATTERNS OF ACTIN GENE REGULATION IN HIGHER PLANTS

Richard Meagher, John McDowell, Shurong Huang, Yong-Quiang An and Elizabeth McKinny, Department of Genetics, University of Georgia, Athens, GA 30602


Plant actins are involved in several fundamental processes essential to growth and development, including positioning of the division plane, cell elongation, programming cell wall development, and cytoplasmic streaming. Plants contain ancient and highly diverse actin gene families. The plant proteins have three times more charged residue variation than animal actin families. Arabidopsis thaliana has nine functional actin genes and one pseudogene which make up six ancient subfamilies. The ACT1/3 subfamily is strongly expressed in leaf, root, and floral primordia and in late pollen development. The ACT2/8 subfamily is expressed constitutively throughout development with the exception of the pollen sack, carpel, hypocotyl and seed coat. The ACT4/12 subfamily is expressed late in pollen development and in root cap. ACT7 is expressed strongly in all tissues of the seedling and developing inflorescence with the exception of the carpel body. ACT11 is strongly expressed in early floral development, most notably the carpel body and developing embryos. Initial data suggest that the gene family encoding the actin binding protein, profilin, is also large, diverse, and differentially expressed. We propose that several actin subsystems arose concomitant with the macroevolution of new organs and tissues early in plant evolution.


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