PAG-II Plant Genome II Conference

Town & Country Conference Center, San Diego, CA, January, 1994.


PG-II: QUANTITATIVE TRAIT LOCI (QTL) MARKERS ASSOCIATED WITH RUMINANT DIGESTION OF BARLEY (HORDEUM VULGARE L.)

QUANTITATIVE TRAIT LOCI (QTL) MARKERS ASSOCIATED WITH RUMINANT DIGESTION OF BARLEY (HORDEUM VULGARE L.).

Laura E. Oberthur, Janice Bowman, and Tom Blake. Dept. of Plant & Soil Sci. and Dept. of Animal Sci., Montana State Univ., Bozeman, MT 59717.


Although most barley grown world-wide is for animal feed, few barleys have been evaluated for livestock utilization. Little is known about specific factors in relation to ruminant feed utilization, thus selection for superior feed quality barleys has not been possible. The high cost involved with animal performance evaluations of barley encourages alternative methods, such as the identification of barley molecular markers associated with animal utilization of grain. The goal of this project is to develop criteria to relate barley genetic markers, as established by the North American Barley Genome Mapping Project, to ruminal dry matter digestion (DMD) and ruminal protein and starch digestion. QTL markers associated with DMD have been identified on the short arm of chromosome 2, near the RbcS gene and on the long arm of chromosome 4, near the isozyme marker iAco2 and RFLP marker ABG500B. The chromosome 4 region also contains gene(s) responsible for some of the variation in soluble (wort) protein. This data in combination with the positive correlation with soluble and ratio of soluble protein to total proteins, suggests that specific proteins may be critical in determining the ability of ruminal microbes to effectively digest barley grain for animal utilization.


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