Plant Genome II Conference
Town & Country Conference Center, San Diego, CA, January, 1994.
PG-II: RICE PHYSICAL AND GENETIC MAP CONSTRUCTION FOR THE ANALYSIS OF
GENOME STRUCTURE
RICE PHYSICAL AND GENETIC MAP CONSTRUCTION FOR THE ANALYSIS OF
GENOME STRUCTURE.
Nori Kurata, Yoshiaki Nagamura, Takuji Sasaki and Yuzo Minobe.
Rice Genome Research Program, NIAR/STAFF, 2-1-2 Kannondai,
Tsukuba 305, Japan
Our aim is to build a highly informative and dense genetic
map linked with a physical map of YAC/cosmid contigs covering at
least 80% of the rice genome size. About 1400 RFLP and RAPD
markers have already been located on our genetic map. RFLP
markers comprise about 890 cDNA clones and 260 genomic clones all
of which have been sequenced. cDNA mapping has resulted in an
expression map, which includes information on tissue-specificity,
distribution of isozyme genes, gene families and functionally
related genes in the genome. RAPD markers generated by the
bulked-segregant method were used to fill gaps of marker-rare
regions on the linkage map. All our markers now cover the entire
genome, with one locus on average at 1.14 cM, i.e. 285 Kb.
Several regions of the genetic map have been converted to
physical maps using YAC clone contigs arrayed by RFLP and RAPD
markers. Gene isolation by map-based cloning, and isolation and
mapping of telomere-associated sequences are discussed. We plan
to use our YAC library, with an average of 400 Kb inserts, to
cover the entire genome as arrayed contigs. The generation of
YAC contigs on four chromosomes, and their relation to the
physical map are presented.
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