Plant Genome II Conference
Town & Country Conference Center, San Diego, CA, January, 1994.
PG-II: THE ISOLATION OF TOMATO GENES USING THE MAIZE TRANSPOSON
ACTIVATOR AND ITS DERIVATIVES
THE ISOLATION OF TOMATO GENES USING THE MAIZE TRANSPOSON
ACTIVATOR AND ITS DERIVATIVES.
Jonathan D G Jones, The Sainsbury Laboratory, Norwich Research
Park, Colney Lane, Norwich NR4 7UH, UK.
Extraction from plant genomes of genes known only by their
Mendelian segregation is still a difficult task. In maize and
Antirrhinum, transposon tagging with endogenous elements has
proved effective for the isolation of such genes. However, in
plants such as Arabidopsis and tomato that lack endogenous
elements, recent successes in the targeted isolation of specific
genes have usually employed map based cloning.
We have been attempting to establish the capacity to isolate
tomato genes by heterologous transposon tagging, by transforming
tomato with binary vector DNA constructs that carry derivatives
of the maize element Activator. Considerable progress has been
made in non-targeted tagging, and also in the targeted tagging of
the tomato dwarf gene and the tomato Cf-9 gene that confers
race-specific resistance to the leaf mould pathogen Cladosporium
fulvum. Recent results will be reviewed.
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