January 15-19, 2011
Town & Country Convention Center
San Diego, CA
Drought is a key environmental factor that affects plant growth and development. MicroRNAs (miRNAs) are gene expression regulators that have been linked to several plant stress responses; however, this relationship is not well-understood. MiRNA expression patterns of drought-resistant Brachypodium in response to shock drought stress were investigated using a plant miRNA microarray platform. Expression was detected of 135 miRNAs in control and 538 miRNAs in stress treated leaf and root tissues. miR1867, miR398, miR528, miR408, miR1450, miR396, miR156, miR166 and miR171 differentially expressed in both tissues. Regulation of miRNAs upon 8 hrs drought stress applications observed by qRT-PCR. Target transcripts of differentially regulated miRNAs were computationally predicted. These findings highlight the presence and thre roles of miRNAs in Brachypoidum under shock drought stress conditions.