Plant & Animal Genomes XIX Conference

January 15-19, 2011
Town & Country Convention Center
San Diego, CA

Fast Genomic Assemblies On A Desktop Computer - E. coli In Less Than A Minute And Human In Less Than A Day

Stephanie Costello¹ , Matther Keyser¹ , Thomas Schwei¹ , Katherine Maxfield¹ , Amber Pollack-Berti, PhD¹ , Jennifer Stieren¹ , Timothy Durfee PhD^1,3 , Richard Nelson PhD¹ , Schuyler Baldwin¹ , Ronda Solberg¹ , Kenneth Dullea¹ , David Frisch PhD^2,3 , Guy Plunkett III PhD^1,3 , Frederick Blattner PhD^1,2,3

¹  DNASTAR, Inc., 3801 Regent Street, Madison, WI 53705, USA

²  Scarab Genomics LLC, 1202 Ann Street, Madison, WI, 53713, USA

³  University of Wisconsin Department of Genetics, Madison, Wisconsin 53706, USA

Advances in high throughput sequencing technology, along with enhanced desktop computing power, provide the average life scientist with the ability to independently process and analyze unprecedented volumes of DNA and RNA sequence data. Researchers now routinely generate numerous gigabytes or even terabytes of data, making access to powerful and easy-to-use software tools essential for analysis and visualization of this data in their own lab. DNASTAR has developed patented software that enables users to align, assemble, and analyze sequence data from virtually all next-generation and third-generation sequencing platforms (Roche 454® Life Sciences, Illumina®, HelicosTM, SOLiDTM, Pacific Biosciences, and Ion Torrent). Utilizing patented alignment algorithms, along with DNASTAR’s SeqMan NGen®, Lasergene® and QSeq® software, scientists can assemble and analyze data from a wide range of sequencing projects including: reference-guided genomic assembly; targeted re-sequencing assembly, SNP discovery, and other variation analyses; de novo genomic, transcriptomic and metagenomic assembly; RNA-Seq analysis; and ChIP-Seq and miRNA epigenomic analysis. DNASTAR recently introduced a new version of SeqMan NGen, which can assemble genomes as large as human on a desktop computer, using DNASTAR’s new patent-pending algorithm, in less than a day. With the introduction of its breakthrough desktop human genome assembler, DNASTAR continues to lead the way with software tools available for life scientists involved with the latest sequencing technologies by providing applications in support of all major next-gen workflows and continuing to improve those workflows to keep pace with continually progressing sequencing technology.