January 15-19, 2011
Town & Country Convention Center
San Diego, CA
LiJie Su , JiangNan Huang , Shuhong Zhao , Mengjin Zhu , Xiaoping Li , Mei Yu
Expression Profile Analyses of Genes And miRNAs in Endometrium of Meishan And Yorkshire Pigs During Early And Mid-gestation
LiJie Su,JiangNan Huang,Shuhong Zhao,Mengjin Zhu,Xiaoping Li, Mei Yu
Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong (Central China) Agricultural University, Wuhan, 430070, PR China
The Chinese Meishan pig is a prolific breed and can farrow three to five more live pigs per litter than the either American or European breeds. In an attempt to understand the mechanisms involved, we used Affymetrix porcine genome Array and miRNA array to investigate the expression changes of pregnancy induced genes and miRNAs in Chinese Meishan and Yorkshire endometria during gestational days 15, 26 and 50. We also combined the expression profiles of genes and miRNAs to identify the miRNA-mRNA regulatory modules during porcine pregnancy. Compared to gestational day 26 and 50, there are the most differentially expressed miRNA-mRNA regulatory modules at gestational day 15 in endometria of Meishan and Yorkshire pigs. The Gene ontology analysis indicated that the predicted targeted genes of these differentially expressed miRNAs may play important roles in various processes, including cell adhesion, extracellular matrix, cell communication and response to hormone stimulus. Of them, the miR-181c was predicted to target the ESR1 gene and the in vivo and in vitro studies are in progress in order to understand the microRNA-mediated regulation of estrogen signaling and response in porcine endometrium. Our study provides novel insights into the differences of endometrial function between Meishan and Yorkshire pigs during early and mid-gestation.