January 15-19, 2011
Town & Country Convention Center
San Diego, CA
Paul J Berkman1 , Adam Skarshewski1 , Michal T Lorenc1 , Kaitao Lai1 , Chris Duran1 , Edmund YS Ling1 , Jiri Stiller1 , Lars Smits1 , Michael Imelfort1 , Sahana Manoli1 , Megan McKenzie2 , Song Weining3 , Marie Kubaláková4 , Hana Simkova4 , Jacqueline Batley2 , Delphine Fleury5 , Jaroslav Doleel4 , David Edwards1
The genome of bread wheat (Triticum aestivum) is predicted to be greater than 16 Gbp in size and consist predominantly of repetitive elements, making the sequencing and assembly of this genome a major challenge. We have reduced genome sequence complexity by isolating wheat group 7 chromosome arms, and applied Illumina paired read sequencing technology and appropriate algorithmic analysis to sequence and assemble low copy and genic regions. Our approach enabled the assembly of all or nearly all genes and low copy regions. The syntenic relationship between wheat, B. distachyon and O. sativa, along with available genetic mapping data, has been used to produce annotated draft syntenic builds whereby the majority of genes have been placed in an approximate order and orientation. These annotated assemblies are maintained on a dedicated public GBrowse database. Our results suggest that the sequencing of isolated chromosome arms can provide valuable information of the gene content of wheat, and that these assemblies can be applied for the identification of candidate genes associated with genetically mapped traits as well as genome wide SNP discovery in this important crop.