PAG-XVIII  Plant & Animal Genomes XVIII Conference

January 9-13, 2010
Town & Country Convention Center
San Diego, CA



W249 : Fruit and Nut Crops


Full Genome Sequencing Of The Woodland Strawberry, Fragaria vesca

Vladimir Shulaev1,25 , Asaph Aharoni 2 , Andrew Allan3 , Pere Arús4 , Tia-Lynn Ashman5 , Nahla Bassil6 , Mark Borodovsky7 , Paul D. Burns7 , Oswald R. Crasta 1 , Ross Crowhurst3 , Jahn Davik8 , Thomas M. Davis 9 , Arthur Delcher10 , Beatrice Denoyes-Rothan11 , Allan Dickerman1 , Amit Dhingra12 , Clive Evans1 , Sergei A. Filichkin13 , Otto Folkerts1 , Jim Hancock15 , Roger Hellens3 , Sarah H. Holt25 , Pankaj Jaiswal13 , Roderick Jensen16 , Schuyler S. Korban 17 , Aaron Liston13 , Alexandre Lomsadze7 , Shrinivasrao P. Mane1 , Lee Meisel18 , Todd P. Michael18 , Ron Mittler 20 , Keithanne Mockaitis21 , Todd C. Mockler13 , Amparo Monfort4 , Henry Priest13 , Juan J. Ruiz-Rojas25 , Steven Salzberg10 , Daniel James Sargent10 , Wilfried Schwab23 , Joao Setubal1 , Herman Silva18 , Janet Slovin24 , Richard Veilleux25 , Riccardo Velasco26 , Roberto Viola26 , Kevin M. Folta 14

1  Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, , Blacksburg, VA 24061, USA
2  Department of Plant Sciences, Weizmann Institute of Science, P.O.B. 26, Rehovot 76100, Israel
3  The New Zealand Institute for Plant and Food Research Limited, Plant & Food Research Mt Albert Private Bag 92169, Auckland Mail Centre, 1142, Auckland, New Zealand
4  Institut de Recerca i Technologia Agroalimenta`ries (IRTA), Centre de Recerca en Agrigeno`mica (CSIC-IRTA-UAB), 08348 Cabrils, Barcelona, Spain
5  Department of Biological Sciences, 4249 Fifth Ave. & Ruskin, University of Pittsburgh, Pittsburgh, PA 15260, USA
6  USDA-ARS, NCGR, 33447 Peoria Rd., Corvallis, OR 97333, USA
7  Wallace H. Coulter Department of Biomedical Engineering, Computational Science and Engineering Division, Center for Bioinformatics and Computational Genomics, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
8  Bioforsk Midt-Norge, Kvithamar, N-7500 Stjordal, Norway
9  Department of Plant Biology, University of New Hampshire, Durham, NH 03824, USA
10  Center for Bioinformatics & Computational Biology, University of Maryland College Park, MD 20742, USA
11  Fruit and Grapevine Research Unit , UREF-INRA, BP 81, 33883 Villenave d’Ornon, France
12  Department of Horticulture & LA and Molecular Plant Sciences Program, Center for Integrated Biotechnology , Washington State University, Pullman, WA 99164, USA
13  Department of Botany and Plant Pathology 3060 Cordley Hall Oregon State University, Corvallis, OR 97331-8530, USA
14  Horticultural Sciences Department, Unifersity of Florida, 1301 Fifield Hall, PO Box 110690, Gainesville, FL 32611, USA
15  Department of Horticulture, Michigan State University, A342C Plant & Soil Sciences Building East Lansing, MI 48824-1325, USA
16  Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
17  Department of Natural Resources and Environmental Sciences, University of Illinois, Urbana, IL 61801, USA
18  Laboratorio de Genetica Molecular Vegetal, Centro de Biotecnología Vegetal Universidad Andrés Bello, Republica 217, 837-0146 Santiago, Chile
19  Department of Plant Biology and Pathology, School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, 190 Frelinghuysen Road, Piscataway, NJ 08854-8020, USA
20  Department of Biochemistry and Molecular Biology, University of Nevada, Mail Stop 200, Reno NV 89557, USA
21  The Center for Genomics and Bioinformatics, Indiana University, 1001 E. 3rd St. Bloomington, IN 47405-7005, USA
22  East Malling Research, New Road, East Malling, Kent ME19 6BJ, UK
23  Biomolecular Food Technology, TU Muenchen, Liesel-Beckmann-Str. 1 (new), 85354 Freising
24  Genetic Improvement of Fruit and Vegetables Laboratory USDA/ARS, 10300 Baltimore Ave, Beltsville, MD 20705-2350, USA
25  Department of Horticulture, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
26  Istituto Agrario San Michele all'Adige, Italy

An international consortium of researchers has used the Roche GS-FLXTM genome sequencing system to generate the complete genome sequence of the woodland strawberry, Fragaria vesca (2n=2x=14). F. vesca is a member of the Rosaceae family, which includes many other well-known fruit crops, such as apple and peach. F. vesca has a favorably small genome (206 Mbp/1C nucleus), is a phylogenetically appropriate model for the octoploid cultivated strawberry, F. ×ananassa, and represents a versatile model for fruit crops due to its short life cycle, transformation efficiency, self-compatibility and abundant seed production. The genome has been assembled into some 3,700 scaffolds with N50 of 1.3 Mb anchored to the strawberry genetic map. Genome annotation has been conducted using ab initio predictions for F. vesca via the 2.51 version of the GeneMark-ES program, TAU, and geneid supplemented homology based annotation using mapping of transcript sequences from cDNAs, gene indices from multiple plant species, and tblastn comparison to UniRef90 (release 15.6). Motifs within sequences for gene models were predicted using InterProScan. Repeats (including LTRs, MITES, and SSRs) were identified. F. iinumae scaffolds have been mapped to F. vesca. All annotation and comparative analysis data have been made available as tracks within the Strawberry Genome Browser using GBrowse 1.69. The strawberry genome project is an example of community-driven sequencing that has benefited from the contributions of consortium participants. The genome sequence data will be an invaluable tool for researchers around the world looking to improve strawberry and other fruit crops through genomics