Plant & Animal Genomes XVIII Conference

January 9-13, 2010
Town & Country Convention Center
San Diego, CA

T. cacao Genome Sequencing

Brian E. Scheffler¹ , Mary V. Duke¹ , Pascal Bouffard² , Tim Harkins³ , Gregory D. May⁴

¹  Genomics and Bioinformatics Research Unit USDA ARS 141 Experiment Station Rd. Stoneville, MS 38776, USA

²  454 Life Sciences, a Roche Company 1 Commercial Street, Branford, CT 06405, USA

³  Roche Applied Science, 115 Hague Road, Bldg. B, Indianapolis, IN, 46250 USA

⁴  National Center for Genome Resources 2935 Rodeo Park Drive East Santa Fe, NM 87505, USA

At the 2009 PAG meeting the Cacao Genome Project (http://www.cacaogenomedb.org/) outlined its general strategy for obtaining a high quality genome of cacao (Theobroma cacao) using complementary mixed sequencing methodologies. During the 2010 PAG meeting a status report of present efforts and findings using second generation sequencing technology will be presented. This includes 10 X genome equivalent (random sheared) using Roche GS-FLX technology and 50 X genome equivalent (short insert 90bp paired ends) using Solexa technology.