January 9-13, 2010
Town & Country Convention Center
San Diego, CA
Senthilvel S1 , Nepolean T1 , Supriya A1,2 , Eshwar K1 , Rajaram V1,3 , Sushil Kumar1,4 , Tom Hash1 , Andrzej Kilian5 , Ram C Yadav2 , Laksmi M Narasu3 , Govind Singh4
Pearl millet [Pennisetum glaucum (L.) R. Brown] is an important component of food security in the semi-arid tropics and assuming greater significance in the context of changing climate and increased demand for highly nutritious food and feed. Molecular tools have been developed and applied in pearl millet on a limited scale. However, the existing tool kit needs to be strengthened further for its routine use in applied breeding programmes. Here we report enrichment of the molecular linkage map of pearl millet by exploiting low-cost and high-throughput Diversity Arrays Technology (DArT) markers. Three existing RIL populations were genotyped using an array of circa 6900 DArT clones developed by PstI/BanII complexity reduction. The number of polymorphic markers scored was 277, 259 and 256 in RIL populations based on crosses ICMB 841-P3 × 863B-P2, H 77/833-2 × PRLT 2/89-33 and 81B-P6 × ICMP 451-P8, respectively. These data were integrated with SSR marker data (117 in ICMB 841-P3 × 863B-P2; 62 in H 77/833-2 × PRLT 2/89-33; and 70 in 81B-P6 × ICMP 451-P8) and individual genetic maps with circa 300 marker loci were constructed. Over 200 markers were mapped in more than one population and the mapping positions of these markers were consistent across maps. Among these, 32 markers representing all 7 linkage groups of pearl millet were mapped in all three populations permitting the development of a well-saturated consensus linkage map of DArT and SSR markers in pearl millet.