January 9-13, 2010
Town & Country Convention Center
San Diego, CA
Cheng-Cang Wu , Rosa Ye , Svetlana Jasinovica , Ronald Godiska , David A. Mead
Bacterial artificial chromosome (BAC) libraries, BAC-based physical mapping, and genome sequencing are critical tools for understanding animal and plant genomes. This knowledge can be used to accelerate breeding and engineering of new animal and plant varieties, exploiting natural genetic diversity to increase yield and improve resistance to biotic and abiotic stresses. BAC libraries built with conventional vectors and methods are inherently biased. Numerous gaps exist in all of the physical and sequencing maps of eukaryotic genomes. We have developed techniques to construct unbiased, randomly-sheared BAC libraries with large inserts (>100 kb) as well as a unique transcription-free BAC vector. Lucigen has provided the genome community with hundreds of Random Shear BAC libraries, constructed from a wide range of bacteria, fungi, worms, nematodes, insects, animals, and plant species. Random Shear BAC libraries provide improved whole genome physical mapping and sequencing, more complete genomic gap closing, more efficient positional cloning, and many other functional genomics capabilites. Here we demonstrate the advantages of the Random Shear BAC cloning process, including colony picking, high density colony filter manufacturing, BAC DNA pooling, BAC library screening, BAC end sequencing, and pooled BAC sequencing on the next-gen platforms. Lucigens Random Shear BAC service is publicly available (see www.lucigen.com), providing the most powerful and convenient tools for genomic researchers.