Plant & Animal Genomes XVII Conference Plant & Animal Genomes XVII ConferenceJanuary 10-14, 2009
Town & Country Convention Center
San Diego, CA
Florent Lefebvre-Pautigny1 , Michel Rigoreau1 , Priyono Priyono2 , Dominique Crouzillat1
Coffea canephora and arabica are coffee species with a major economic interest which is also considered as an important raw material for food industry. Therefore this crop species are considered as an orphan crop due to the lack of investments and scientific research from the international community. Among the main priorities the establishment of a core genetic map for C. canephora is certainly of main interest due to high level of diversity in this crop species. The establishment of a high density genetic map on C. canephora using mainly SSR and SNP markers was focused on automation of PCR markers using high resolution melting (HRM) technology. The use of HRM for SNP genotyping allows an exhaustive detection of all the SNPs carried on the PCR amplification product facilitating the transposition of the SNPs among different mapping progenies. The detection of SSR polymorphism is more efficient than gel based technologies since the DNA variability is detected immediately after the PCR run without any further step. HRM reliability was estimated using traditional SNP genotyping with TaqMan probe and SSR fluorescent labelling. The results clearly indicate that the HRM method can be used efficiently to detect SNPs and microsatellites DNA polymorphisms in out crossing progenies such as Coffea canephora. The resulting coffee genetic map includes more than 700 SNPs and SSRs that are easily transposable on different progenies. This set of markers facilitates the establishment and management of QTLs in a global strategy for coffee marker assisted selection.