Plant & Animal Genomes XVI Conference Plant & Animal Genomes XVI ConferenceJanuary 12-16, 2008
Town & Country Convention Center
San Diego, CA
Anne Y. Fennell , Kathy Mathiason , Dong He , Grimplet Jérôme
In grapevines bud dormancy is an adaptive strategy for the survival of drought, high temperature, low temperature and freeze dehydration stress. In the North American grape Vitis riparia, short photoperiod promotes growth cessation and bud endodormancy independently of temperature influence. Gene expression has been monitored (custom cDNA and Affymetrix Vitis GeneChip microarrays) during photoperiod induction of dormancy and chilling requirement fulfillment to identify phase specific patterns of gene expression during endodormancy induction and release. In addition, bud and shoot tissue comparisons have been used to identify paradormancy related gene expression. Paradormant buds and shoot tissue show distinctly different hormone related gene expression patterns (transport and metabolism). During dormancy induction, increased expression related to bud development (cell wall and floral development genes) as well as increased expression of unknown genes has been observed. During chilling fulfillment, there is significant down regulation of gene expression (metabolism, cell defense, and stress response related genes). While down regulation of many of these genes continues in ecodormant buds, significant up regulation (transport, signaling, and information processing) coincides with chilling fulfillment. Photoperiod controlled initiation of endodormancy and precisely timed chilling studies are being used to further define functional relationship of expression patterns to dormancy cycle.