Plant & Animal Genomes XV Conference Plant & Animal Genomes XV ConferenceJanuary 13-17, 2007
Town & Country Convention Center
San Diego, CA
Marcos A. Machado , Alexandre Morais Amaral , Juliana Freitas Astua , Irving Joseph Berger , Raquel Luciana Boscariol Camargo , Eduardo Fermino Carlos , Helvecio Della Coletta Filho , Eliane Cristina Locali , Adriano Reis Lucheta , Dagmar Ruth Stach Machado , Ana Carolina Basilio Palmieri , Dario Abel Palmieri , Luis Peroni , Ana Carla Silva Pinhati , Valdenice Moreira Novelli , Marcelo Silva Reis , Alessandra Alves Souza , Marco Aurelio Takita , Maria Luisa Penteado Natividad Targon , Mariangela Cristofani Yali
The sequencing of citrus ESTs in Brazil produced a huge number of information on expressed genome. The CitEST database includes over than 300,000 reads with 260,000 valid reads used for assembly and comparison. Unigenes set of sweet orange (32,121), mandarin (18,873), Poncirus trifoliata (12,873) and other citrus species (27,954) are generated from several libraries under biotic (Xylella fastidiosa, CTV, CiLV, Phythophtora, mite), abiotic (drought) stress and during fruit development. The bioinformatics pipeline includes sequencing processing, unigenes building, peptide and protein extraction, annotation of sequence data and in silico hybridization. A general unigene - unigene and codon usage comparison between different species (Arabidopsis, rice, grape, maize and Populus), besides within citrus species was carried out. Detailed analysis among libraries was performed.