Plant & Animal Genomes XV Conference

January 13-17, 2007
Town & Country Convention Center
San Diego, CA

Construction Of Physical Map For Brachypodium distachyon

Ming-Cheng Luo¹ , Yaqin Ma¹ , Naxin Huo^1,2 , John Vogel² , Gerard R. Lazo² , Teresa Hill² , Devin Coleman-Derr² , Daniel Hayden² , Jan Dvorak¹ , Olin Anderson² , Frank M. You^1,2 , Yuqin Hu¹ , Charles X. Wang¹ , Yong Q. Gu²

¹  Department of Plant Sciences, University of California, Davis, CA 95616, USA

²  USDA/ARS,WRRC, 800 Buchanan Street, Albany, CA 94710, USA

Brachypodium distachyon is a newly established model system for temperate cereal (including wheat and barley) and other grasses because of its compact genome size (355Mb) and a range of biological features such as small physical size, short life cycle, and undemanding requirements. To develop genomics tools for this emerging model species, two bacterial artificial chromosome (BAC) libraries constructed from B. distachyon BD-21 genomic DNAs, each representing 10x genome equivalent, are fingerprinted using SNaPshot-based fingerprinting procedure, with ABI3730XL platform and GS1200 size standard. An initial assembly of HindIII BAC library includes 26,967 BAC clones, after removing small insert clones and cross-contaminated clones, which is 75% of total clones fingerprinted for the library. There are currently 705 contigs and 1987 singletons. Largest contig is ca. 3 Mb and average contig is 0.47 Mb in length. Total unique CB (consensus band) units accounts for approximate 325 Mb or 92% of the B. distachyon genome. The completion of B. distachyon physical map integrated with BAC end sequence (BES) data will provide genome-wide resources of Brachypodium to serve the research community for sequence assembly, comparative genome analysis, gene isolation, and functional genomics analysis.