Plant & Animal Genomes XV Conference

January 13-17, 2007
Town & Country Convention Center
San Diego, CA

Progress Of Musa (Banana And Plantain) Genetic Diversity And Genome Analysis At Agropolis-CIRAD

Isabelle Hippolyte¹ , Frederic Bakry¹ , Francoise Carreel¹ , Christophe Jenny¹ , Elizabeth Arnaud² , Nicolas Roux² , Jean-Christophe Glaszmann³ , Ange-Marie Risterucci³ , Olivier Garsmeur³ , Angelique D'Hont³ , Franc-Christophe Baurens³

¹  CIRAD, UR75, Avenue Agropolis, Montpellier, F-34398 France

²  Inibap, Bioversity-France, Parc Scientifique Agropolis 34397 Montpellier Cedex 5, France

³  CIRAD, UMR 1096 AgroM-Cirad-Inra, Avenue Agropolis, Montpellier, F-34398 France

Musa has been the subject of diverse studies at CIRAD since the mid 1980s with the advent of biochemical and molecular markers. In the context of the Generation Challenge Programme, these have been pursued with heightened intensity through a network of international interactions. Here we report surveys of SSR markers for germplasm diversity analysis and genome mapping. A set of 500 accessions chosen to represent the species was characterized with 12 SSRs. Among them, 182 were also genotyped with DArT markers (see E. Huttner et al., speaker in this workshop). This contributes to a comprehensive understanding of Musa diversity. Simultaneously, the progeny of a cross between two heterozygous parents (Borneo x Pisang liling) are being genotyped using 150 SSR markers; the preliminary map will be presented. More recently, the production of two BAC libraries has been undertaken using a doubled haploid regenerated from anther culture of the clone Pahang. Altogether, these genomic resources will contribute to the development of a coordinated initiative of fine genome analysis to support Musa breeding.