Plant & Animal Genomes XV Conference

January 13-17, 2007
Town & Country Convention Center
San Diego, CA

Application Of Diversity Arrays Technology (DArT„§) For Sorghum Mapping, Diversity Analysis And Breeding

Emma S Mace¹ , Andrzej Killian² , Kirsten Halloran¹ , Ling Xia² , Bertrand Collard¹ , David R Jordan¹

¹  1Hermitage Research Station Department of Primary Industries and Fisheries MS 508 Warwick, Qld 4370 Australia

²  Diversity Arrays Technology Pty Ltd, Building 1 CSIRO Forestry 1 Wilf Crane Crescent, Yarralumla PO Box 7141, Yarralumla, ACT 2600, Australia

The sequential nature of gel-based marker systems entails low throughput and high costs per assay. Commonly used marker systems such as SSR and SNP are also dependent on sequence information. These limitations result in high cost per data point and significantly limit the capacity of breeding programs to obtain sufficient return on investment to justify the routine use of marker-assisted breeding for many traits and particularly quantitative traits. Diversity Arrays Technology (DArT) is a cost effective hybridisation-based marker technology that offers a high multiplexing level while being independent of sequence information. This technology offers sorghum breeding programs an alternative approach to whole-genome profiling. We report on the application and usefulness of DArT genotyping for mapping 2 populations of sorghum; 90562xISCV745 and IS8525x31945-2 and its likely application in a range of diverse and adapted sorghum germplasm.