International PAG - Solexa Workshop


Tuesday - Late Afternoon, 16 January 2007 --- 3:50 pm - 6:00 pm

Solexa Workshop Golden West

Organizer:
Liz Lim, Solexa

(elim@solexa.com)

Speakers:

Pam Green
Insights from deep small RNA profiling

Application of our novel high throughput sequencing technology using CSMA® for whole genome re-sequencing, efficient SNP discovery and genome structure elucidation

Christian D. Haudenschild, Ph.D.,
Senior Manager, Technical Accounts
Solexa, Inc.

Many competing approaches to characterize an organism's genome and transcriptome exist. Most of these technologies however have strong limitations either due to cost, sensitivity or ease of implementation on a large scale. With this in mind, Solexa Inc. has developed the next generation of high throughput, low cost, DNA sequencing machines. The major advancement comes from the use of the CSMATM (clonal single molecule array) technology allowing the simultaneous amplification of millions of single molecules randomly scattered on a glass surface. The resulting DNA clusters can then be sequenced in parallel, one base at a time, using four proprietary reversibly terminated fluorescent nucleotides. The sequencing apparatus is able to follow base-by-base integration of these labeled nucleotides to reconstruct the sequence of each individually amplified DNA molecule. Sequencing on a single device of around 1 billion bases in a few days at a fraction of the usual cost can thus be achieved. This enables applications in the field of whole genome re-sequencing, whole transcriptome sequencing, high precision gene expression tag profiling and epigenome interrogation. This ability to rapidly produce tens of millions of short sequences (20-35 mers) can also be used for a whole range of diverse applications including methylation and chromosomal rearrangement mapping.

Digital Analysis of the Transcriptome

Gary P. Schroth, Ph.D.,
Director, Expression Applications R&D
Solexa, Inc., 25861 Industrial Blvd Hayward, CA 94545

We have used our Clonal Single Molecule ArrayTM sequencing technology to study the transcriptome in a range of biological samples. This technology has been used to generate quantitative, digital profiles for both mRNA and microRNA. Since this approach does not require a priori knowledge of the genome, it can used to study complete expression in any organism. We will show specific examples of novel transcription that has been discovered using the Solexa 1G Genetic Analyzer.


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This page last updated Friday, 12-Jan-2007 18:27:16 EST