Plant & Animal Genomes XIV Conference

January 14-18, 2006
Town & Country Convention Center
San Diego, CA

Comparative Genomics Of B. oleracea And Its Allies: Integrating BAC Hybridization Data

Manuel J. Torres , A. F. Feltus , John E. Bowers , Andrew H. Paterson

  Plant Genome Mapping Laboratory, 111 Riverbend Rd, University of Georgia, Athens, GA 30602 USA

The Brassicaceae (or Cruciferae) is a dicot family comprised of 13 tribes with a total of 360 genera including valuable crop plants in the genus Brassica and the plant model Arabidopsis thaliana. Comparative genome analysis between Brassica crop plants and Arabidopsis is facilitated by the complete annotation of the genome sequence of Arabidopsis and detailed information about map positions, gene copy numbers, intergenic sequences, and duplicated chromosomal segments. We implemented CMap for providing a visually informative image of three genetic maps of Brassica spp. (Lan, DelMonte et al. 2000; Babula, Kaczmarek et al. 2003; Li, Gao et al. 2003; Udall, Quijada e al. 2005). Probes used to construct the genetic maps, some from Arabidopsis cDNAs and others from Brassica genome survey sequences, were localized to map positions on the Arabidopsis genome sequence. We used ~600 of these sequences, together with Brassica homologs for 1127 low-copy Arabidopsis sequences, to design 1729 overgo probes for hybridization studies with 3 Brassica BAC libraries. We have constructed an ‘alignment’ of the Brassica chromosomes with those of Arabidopsis and will integrate the hybridization data from the set of overgo probes to order the Brassica BAC libraries.