Plant & Animal Genomes XIV Conference

January 14-18, 2006
Town & Country Convention Center
San Diego, CA

Discovery And Characterization Of Disease Resistance Gene Homologues In A Plant Transformation Competent BiBAC Library Of The Banana Musa acuminata cv. Tuu Gia (AA)

ANDREW C. JAMES , Raul Jimenez-Martinez , Blondy Canto-Canche , Santy Peraza-Echeverria

  Centro de Investigacion Cientifica de Yucatan, Calle 43 # 130, Col. Churburna de Hidalgo, Merida C.P. 97200, Yucatan, Mexico

The aim of this work is to develop a BIBAC collection containing disease resistance homologues ready to be used in genetic complementation experiments. Currently, we are isolating by degenerate PCR different classes of resistance gene homologues from the banana cv. Tuu Gia (AA) that can be used as probes to screen the BIBAC library. In this study, we report the isolation and characterization of BIBAC clones containing homologues of the Pto serine threonine protein kinase class of resistance genes. The BIBAC library was screened by a combination of pooling PCR and colony blot hybridization. Several clones were identified and partially sequenced to confirm their identity as Pto homologue sequences. With these results we have made the first step towards the generation of a broad BIBAC collection containing banana resistance gene homologues organized either as gene clusters or as singletons