Plant & Animal Genomes XIV Conference

January 14-18, 2006
Town & Country Convention Center
San Diego, CA

Geographic Origin And Hybrid Haplotype Of Musa Revealed By High Resolution Genotyping Of The Cytoplasmic Genomes

Boonruangrood Ratri , Desai Dhairyasheel , Silvia Fluch , Kornel Burg

  ARC Seibersdorf research gmbh, Biogenetics, Seibersdorf, 2444, Austria

The Musa genome is constantly expanding with its high rate of somaclonal variations. The current classification based on morphological characters and predicted genomic constitution after crossing over makes this system unreliable for sterile, heterozygous and polyploid Musa accessions. Due to its uniparental inheritance cp and mtDNA provides an interesting and comprehensive tool for parental analysis of plants. In this study we have used cpDNA and mtDNA PCR-RFLP marker system revealing hybrid origins and geographic distribution of 48 Musa accessions. Phylogenetic analysis provided the clear distinction between A and B cytoplasm and relationships between their polyploid progenies. Additionally the acuminata genotypes could be separated into two geographically separate groups, one representing genotypes of India, Thailand, Malaysia and Indonesia, while Philippines and Papua New Guinea formed the other group. The organellar genome analysis of Musa showed that the use of conserved gene markers for cp and mtDNA analysis is not only useful in determining inter and intraspecific variation but also for confirming the genetic and geographic origin of the cytoplasmic genomes in the hybrids.