Plant & Animal Genomes XIV Conference

January 14-18, 2006
Town & Country Convention Center
San Diego, CA

Building The Physical Map Of The Cabernet Sauvignon Genome

Sophie Paillard¹ , Sarah Tual¹ , Isabelle Le Clainche¹ , Aurélie Canaguier¹ , Corinne Cruaud² , Carole Dossat² , Patrick Wincker² , Vincent Thareau³ , Fabrice Legeai⁴ , Jean Weissenbach² , Anne-Françoise Adam-Blondon¹

¹  Unité de Recherches en Génomique Végétale (URGV), UMR INRA-CNRS-Université d'Evry, 2 rue Gaston Crémieux, BP5708, 91057 Evry-cedex, France

²  Génoscope, 2 rue Gaston Crémieux,BP5708, 91057 Evry-cedex, France

³  present address: Institut de Biotechnologie des Plantes, Université Paris-Sud, Bât. 630, 91405 Orsay cedex, France

⁴  Unité de Recherches en Génomique-Informatique, Infobiogen, 523 place des Terrasses, 91034 Evry cedex, France

A number of genetic traits are crucial for wine production including quality traits as well as pest resistance. However, the classical analysis of these traits is slowed down by the long generation time of this species. The relatively small size of the the Vitis vinifera genome (470 Mb) compared to other crops makes it an attractive species for genomic approaches. In order to construct a physical map of the grape genome, a 13 genome equivalents BAC library (45,000 clones, 145 kb mean insert size) from the grapevine cultivar Cabernet Sauvignon N was constructed a few years ago (Adam-Blondon et a.l, 2005, Theor Appl Genet, 110 : 1363-1371) and was chosen as a resource for the International Grape Genome Project (IGGP). The fingerprints of a set of 30,000 clones (9 genome equivalents) were obtained according to the protocol published by Luo et al. (2003, Genomics, 82 : 378-389) and adapted to grape by Moroldo et al. (2004, 7th Int. Symp. on Grapevine Physiology and Biotechnology). The assembly was performed with FPC (Soderlund et al., 2000, Genome Research, 10 : 1772-1787) and comprises less than 2,000 contigs. Around 1,300 markers have been anchored on this map, among which 241 are genetically mapped. In order to improve the anchoring of the contigs on the genetic map, the BAC end sequences (77,237 BES) are being used to develop polymorphic markers that will be both anchored on the physical map and mapped genetically. This physical map is already used to speed up the mapping of candidate genes and to improve the mapping of QTLs for different traits of agronomic interest as preliminary steps for their positional cloning.