Plant & Animal Genomes XIV Conference

January 14-18, 2006
Town & Country Convention Center
San Diego, CA

Assembly Of Chloroplast Genomes For Pinus taeda And Liriodendron tulipifera

John E. Carlson¹ , James H. Leebens-Mack² , Daniel G. Peterson³

¹  School of Forest Resources and Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA

²  Department of Biology and Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802

³  Department of Plant and Soil Sciences, 117 Dorman Hall, Box 9555, Mississippi State University, Mississippi State, MS 39762

Draft chloroplast genome sequences will be reported for loblolly pine and yellow poplar. The chloroplast genomes for loblolly pine and yellow poplar were assembled from genomic DNA sequences gathered by the high throughput GS20 DNA sequencer from the 454 Life Sciences Company. Approaches to quickly obtain and assemble chloroplast genome sequences from total DNA and from BAC libraries using the GS20 DNA sequencer will be presented. The structure of the Pinus taeda chloroplast genome will be compared to the previously sequenced chloroplast genomes for Pinus koraiensis and Pinus thunbergii and to the chloroplast genomes of basal angiosperms including Liriodendron. Comparisons of synonymous and nonsynonymous substitution rates across protein-coding genes and across taxa will be also presented.