Plant & Animal Genomes XIV Conference

January 14-18, 2006
Town & Country Convention Center
San Diego, CA

Proteome Analysis Of Aluminum Tolerance In Arabidopsis: Identification Of Major Proteins From Two-Dimensional Gels (pI 4-7)

Chung Sun Kim¹ , Owen A. Hoekenga² , Gung Pyo Lee³

¹  Institute of Life Science & Natural Resources, Korea University Life & Environmental Science Building, Korea University 5-1, Anam-dong, Sungbuk-gu, Seoul 136-701 Korea (Republic of)

²  Boyce Thompson Institute for Plant Research 321 Federal Nutrition Lab Cornell University Ithaca, NY 14853-2901, USA

³  Dept. of Applied Plant Science, College of Industrial Science, Chung-Ang University Naeri, Daedukmyeon, Kyeongkido 456-756, Korea (Republic of)

Aluminum (Al) toxicity, which is caused by the solubilization of Al3+ in acid soils resulting in inhibition of root growth and nutrient/water acquisition, is a serious limitation to crop production, because up to one-half of the world's potentially arable land is acidic. Two-dimensional (2-D) gel electrophoresis and mass spectrometry were applied to identify water-soluble proteins in extracts of Arabidopsis roots (Col-0 and Landsberg erecta) and to follow their responses at the time course of 1, 2, and 3 days under aluminum stress (50mM AlCl3). About 100 spots of pI 4-7 were detected on 2-D gels by colloidal Coomassie Brilliant Blue staining. Differentially expressed about 30 spots were selected for in-gel digestion followed by Maldi-TOF analysis. Peptide matching and protein analysis were conducted by NCBI nonredundant protein database using ProFound program. These include housekeeping enzymes, hydrolytic enzymes, defence- and stress-related proteins such as fructose-bisphosphate aldolase, aldo/keto reductase family protein, 1-aminocyclopropane-1-carboxylate oxidase, alcohol dehydrogenase, GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase, Ras-related GTP-binding nuclear protein (RAN-2), and monodehydroascorbate reductase. We will discuss the differentially detected 11 spots in the 2-D gel pattern changes in Col-0 and Ler during 1 to 3 days