Plant & Animal Genomes XIV Conference Plant & Animal Genomes XIV ConferenceJanuary 14-18, 2006
Town & Country Convention Center
San Diego, CA
Wing Y. Cheung , Dahu Chen , Christiane Menard , Sophie Bernier , Benoit S. Landry
Single nucleotide polymorphism (SNP) markers are the most abundant high throughput (HT) DNA markers and have the ability to detect single nucleotide differences in alelles that cannot be detected by other marker systems. The combined potential for very high density marker-based maps together with the potential for very high throughput analysis indicates that SNPs will be the markers of choice for genetic mapping, DNA fingerprinting and marker-assisted breeding (MAB) in the future. A large number of single nucleotide polymorphism SNP markers have been developed for corn both in the private and the public sectors. With these resources available, it is therefore time to find efficient and cost-effective ways to put HT genotyping with corn SNP markers into applications.
The SNPlex™ Genotyping system of Applied Biosystems has the ability to multiplex 48 SNPs in a single genotyping reaction and hence reduce the cost of using SNPs as genetic markers in large scale genotyping projects. As a pilot project, we have examined the use of the SNPlex system for genotyping public corn SNPs in genetic mapping using segregating population, germplasm fingerprinting and marker-assisted backcross selection.