PAG-XIV  Plant & Animal Genomes XIV Conference

January 14-18, 2006
Town & Country Convention Center
San Diego, CA


Poster: Microarrays

P767

Gene Expression Changes In The Spleen After Salmonella enteritidis (Se) Challenge Of F8 Advanced Intercross Lines (AIL) Of Chickens

Huaijun Zhou1, 3 , Dan Nettleton2 , Susan J. Lamont1

1  Department of Animal Science, Iowa State University, Ames, IA 50011, USA
2  Department of Statistics,Iowa State University, Ames, IA 50011, USA
3  Food Research Program, Agriculture and Agri-Food Canada, Guelph, ON N1G 2V7, Canada

Global gene expression after SE challenge was profiled with chicken 13K cDNA microarrays (Fred Hutchinson). Spleens of two F8 AIL (broiler by Leghorn; broiler by Fayoumi), harvested at 7 or 8 days after SE inoculation, were used to quantify SE burden and isolate mRNA. Within each AIL and harvest time, the 20% high and 20% low SE-burden birds, and unchallenged birds, were designated the three “treatment” groups. Two replications of a loop design with direct-comparisons between treatments were used within each AIL and time. Gene signal intensity was globally normalized by LOWESS and expressed on natural log scale. A mixed model including line, treatment, time, array (random effect), dye, and all interactions among treatment, time, and line was used to identify differentially expressed genes between treatments, at the 1% significance level. There were four tests (two AIL, two times) per gene for each paired comparison. Candidate genes, both statistically (this study) and biologically (literature) included: interleukin-1 beta, B-G antigen, NK-tumor recognition protein, interleukin-10 receptor 2, Toll-like receptor 2 type 1 precursor, CD3 epsilon, p53-related protein, Tumor suppressor p53-binding protein 1, Interleukin-1 receptor-associated kinase-2, chemokine ah221, chemokine ah294, T cell receptor delta chain, CC chemokine receptor 6, tumor necrosis factor receptor, MHC class II histocompatibility antigen B-L beta chain 2, Tumor necrosis factor receptor superfamily member 16 precursor, Interferon regulatory factor 1, caspase-1, lymphocyte-specific protein 1, TNF alpha-inducible ATP-binding protein, NF-kappaB inhibitor alpha. These differentially expressed genes are candidates for detailed hypothesis-driven investigation of genes determining resistance to SE in chickens.