PAG-XIV  Plant & Animal Genomes XIV Conference

January 14-18, 2006
Town & Country Convention Center
San Diego, CA



Poster: Large Insert Libraries


P49

DNA Library And Service Resources For Plant And Animal Genomics

Michael Atkins , Jeanice Troutman , Monica Munoz-Torres , Stephen Ficklin , Eric Fang , Barbara Blackmon , Christopher Saski , Jeffrey Tomkins

  Clemson University Genomics Institute (CUGI), Rm 310 BRC, 51 New Cherry Rd, Clemson, SC 29634, USA

Clemson University houses a Genomics Institute (CUGI) focusing on research, teaching, and service in genomics. One of the primary service components of CUGI is to produce and distribute high quality BAC and EST libraries from plants, animals, and microbes and to provide convenient and affordable access to these resources. This service unit within CUGI is called the BAC/EST Resource Center (BRC). The CUGI BRC sells library related products on a cost-recovery basis via a secure web site at www.genome.clemson.edu. BRC products for sale include high-density colony filter arrays, individual clones and whole libraries. With the assistance of high-throughput robotic devices, CUGI has produced, acquired and distributed a significant amount of the plant, insect and microbial BAC libraries in use today. Over the past year, we have constructed a number of new BAC libraries for a variety of plant, insect, and microbial genomes and presently maintain in excess of 10 million clones. In addition to the distribution of library-related products, the CUGI offers a wide variety of genomic services which include BAC fingerprinting using the new high information content fingerprinting (HICF) technology and a variety of DNA sequencing methodologies (BAC-end, EST, shotgun, finishing/gap cplosure). DNA library construction services include BAC, shotgun and EST library construction. The addition of a proteomics facility at CUGI provides services in shotgun proteomics, protein characterization/quantification, protein sequencing, and analysis of protein modification (glycosylation, phosphorylation, truncation, etc). The CUGI Proteomics Facility also has the capacity to analyze a wide variety of small molecules and secondary metabolites providing valuable information for a wide diversity of basic and applied research applications.