Plant & Animal Genomes XIV Conference

January 14-18, 2006
Town & Country Convention Center
San Diego, CA

Generation And Annotation Of Large-Scale Expressed Sequence Tags (ESTs) From A Full-Length Enriched cDNA Library Of Porcine Adipocyte

Tae-Hun Kim¹ , Nam-Sun Kim² , Kyung-Tai Lee¹ , Jung-Hwa Oh² , Hye-Sook Park¹ , Gil-Won Jang¹ , Bong-Hwan Choi¹ , Hae-Young Lee¹ , Hee-Bal Kim³

¹  Division of Animal Genomics & Bioinformatics, National Livestock Research Institute, Rural Development Administration, Omokchun-dong 564, Kwonsun-gu, Suwon 441-706, Korea.

²  Laboratory of Human Genomics, Genome Research Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon 305-333, Korea

³  School of Agricultural Biotechnology, Seoul National University San 56-1, Sillim-dong, Gwanak-gu, Seoul 151-742, Korea

Genome research in farm animals will expand our basic knowledge of the genetic control of complex traits, and the results will be applied in the livestock industry to improve meat quality and productivity as well as reduce the incidence of disease. A combination of quantitative trait locus mapping and microarray analysis is a useful approach to reduce the overall effort needed to identify genes associated with quantitative traits of interest. We constructed a full-length enriched cDNA library from porcine adipocyte. The average size of the cDNA inserts and the fullness ratio of cDNA were estimated to evaluate the quality of the library. Approximately 15,000 clones were randomly selected, sequenced. These ESTs were clustered and assembled to obtain unique sequences. The unique sequences were categorised using terms from the GO database. Since a large portion of these pig sequences has not yet been annotated, gene annotation was performed by extracting information with the term already annotated. Putative novel transcripts resulted after comparison and filtering with the TIGR SsGI; these included a large percentage of singletons and a small proportion of contigs. In conclusion, these sequence data generated in this study will provide valuable information for studying the expression profiles by EST-based microarrays and will assist in the condensation of current pig TCs into clusters representing longer stretches of cDNA sequences. The isolation of genes expressed in backfat tissue is the first step toward a better understanding of backfat tissue on a genomic basis.