Plant & Animal Genomes XIV Conference Plant & Animal Genomes XIV ConferenceJanuary 14-18, 2006
Town & Country Convention Center
San Diego, CA
Gerard R. Lazo , Yong Q. Gu , Frank M. You , Curt C. Crossman , Devin Coleman-Derr , Olin D. Anderson
Sequencing technology and the tools by which to process information have improved tremendously over recent years. It is the combination of these technologies which help in adapting protocols by which to sequence complex genomes. Methods of sequencing range from random shotgun sequencing approaches using now traditional capillary electrophorsis methods to full genome sequencing using picolitre pyrosequencing approaches. Regardless of the methods used, highly repetitive regions of the genome can make it difficult to close gaps in attempts to build contiguous sequence data. A now common method used to prepare sequencing of the genome is to map out a sequencing scaffold from large-insert genome libraries using fingerprint profiling techniques, sometimes referred as high information content fingerprinting (HICF), and assembly of the scaffolds by software (Luo et al., 2003). A similar methodology was used in the design of a sequencing strategy, applying the fingerprint strategy to smaller subclones and using labels to restriction fragments cut with enzymes of four base recognition sites. The fingerprint profile data was extracted with GenoProfiler (You et al., 2004) software, and assembled with FPC (Soderlund et al., 2000). The method helped to reduce the number of sequences needed to complete sequencing of a BAC clone, normally sequenced using random shotgun sequencing approaches until complete coverage was apparent. We have tested this method on BAC clones derived from the wheat genome, well known for its complex genome structure.