Plant & Animal Genomes XIV Conference

January 14-18, 2006
Town & Country Convention Center
San Diego, CA

Identification And Functional Annotation Of Unique EST Sequences Between Vitis shuttleworthii And V. vinifera Grapes

Jiang Lu¹ , Hong Huang¹ , Wayne Hunter² , Phat Dang²

¹  Center for Viticulture and Small Fruit Center Center for Viticulture and Small Fruit Research, Florida A&M University, 6505 Mahan Drive, Tallahassee, FL32317

²  USDA-ARS, USHRL, Fort Pierce, FL 34945

In addition to the approximate 190,000 ESTs from about 70 different cDNA libraries of the European grapes (Vitis vinifera), ESTs from several north American grape species, including V. shuttleworthii, V. aestivalis, V. raparia, and V. rotundifolia have been generated during last few years. The largest EST data set among this group was generated from V. shuttleworthii, one of the most disease and pest resistant grape species originated and distributed in the southeast United States. We have sequenced 18,000 ESTs from a single cDNA library derived from mixed tissues of V. shuttleworhtii. Percentage of new genes derived from the ESTs decreased as the number of EST increased, but on top of the 18,000 ESTs, there were still twenty plus percent of new unigenes being discovered. A total of 8,500 unigenes were generated from the single V. shuttleworthii library. A comparative genomics analysis between V. shuttleworthii and V. vinifera grapes was conducted. When compared to the V. vinifera ESTs and unigenes, 3,478 ESTs and 2,140 unigenes of V. shuttleworthii did not find homologies (1E-20) to the 190,000 plus ESTs generated from V. vinifera grape. Functional annotation indicated that high percentage of these unmatched V. shuttleworthii ESTs are associated with disease resistance /stress tolerance.