Plant & Animal Genomes XIV Conference

January 14-18, 2006
Town & Country Convention Center
San Diego, CA

Development Of Microsatellite Markers In An Estuarine Opossum Shrimp, Americamysis bahia (Crustacea: Mysida)

Ruth A. Bagnall¹ , Rosemarie C. Rosell¹ , Laura Boykin² , Phat Dang² , Cindy McKenzie² , Donald R. Frohlich¹ , Robert G. Shatters Jr.²

¹  Department of Biology, University of St. Thomas, 3800 Montrose Blvd., Houston, TX, 77006, USA

²  USDA, ARS, Horticultural Research Laboratory, 2001 South Rock Rd., Fort Pierce, FL, 34945, USA

Americamysis bahia is a sensitive, and EPA approved, indicator of estuarine water quality. Genomic DNA, prepared from 200-300 whole adults, was cut and size selected for 400-900 nt. After addition of linkers and PCR enrichment, products were probed with biotinylated di- and tetra- nucleotide probes, (CA)16, and (GATA)8. Streptavidin coated paramagnetic beads were then used to bind hybridized sequences and repetitive DNAs were isolated and concentrated after denaturation. After a second PCR enrichment, putative microsatellites were TA cloned into a P-Gem vector and positive transformants were picked from a blue/white selection system. Herein, we report microsatellite sequences from A. bahia that may be useful in characterizing populations along the N. American Gulf and Mexican coasts.