Plant & Animal Genomes XIV Conference

January 14-18, 2006
Town & Country Convention Center
San Diego, CA

Use Of A New High-Density Size Standard For Sizing Across Multiple Fragment Analysis Applications

Larry Joe , Joan Hauser , Surekha Karudapuram , Carmen Cesena , Ariana Wheaton , Cheryl Heiner , Shiaw-Min Chen

  Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA, 94404

A high performance size standard is essential for many fragment analysis applications, including AFLP®, VNTR, STR, BAC fingerprinting, and SNP analysis. Using the Applied Biosystems 5th dye technology, we have developed a new high-density size standard with larger sizing range and no peak migration anomalies. This new size standard provides improved sizing precision over a wide sizing range that span both small and large fragment sizes. In this presentation we will describe our new size standard and illustrate its use with a variety of fragment analysis applications.